Occurrence, morphological characterization and antigen localization of felid herpesvirus-induced pneumonia in cats: a retrospective study (2000-2006).

The pathological findings in nine cases of pneumonia associated with infection by felid herpesvirus-1 (FeHV-1) are presented. The animals affected were five kittens, two subadult cats and two adult cats. The microscopical changes included fibrinonecrotic pneumonia and severe necrosis of the bronchial and bronchiolar epithelium. FeHV-1 antigen was detected immunohistochemically in pneumocytes, alveolar macrophages and necrotic bronchial and bronchiolar epithelial cells. In the youngest kitten viral antigen was also found in the liver. FeHV-1 infection should therefore be considered as a differential diagnosis in cats with fibrinonecrotic pneumonia and demonstration of viral antigen by immunohistochemistry is a useful diagnostic procedure in such cases.

Spontaneously occurring alimentary osteofluorosis associated with proliferative gastroduodenopathy in rabbits.

Growing rabbits from two rabbitries, fed with commercial concentrates and hay, developed painful thickenings of the extremities. Four rabbits from each farm were clinically examined and necropsied. All animals showed multiple moderate to severe osseous proliferations of extremities and mandibles and a mild to severe proliferative gastroduodenopathy. Histologically, periosteal and endosteal hyperostosis and a mild to severe proliferation of the gastric and duodenal mucosa were noted. Bone analyses revealed 12,700 and 15,000 microg fluoride per gram of bone ash in affected rabbits, compared with 550 microg fluoride in a control animal. A highly elevated fluoride content was found in concentrates. Vitamin A levels were moderately increased only in one concentrate, and copper levels were normal. Results indicate that alimentary fluoride intoxication caused prominent bony proliferations in the examined rabbits. Whether the proliferative gastroduodenopathy is related to the elevated fluoride intake or represents an incidentally occurring secondary disease remains to be determined.

A human case of travel-related rabies in Austria, September 2004.

A young male Austrian tourist, aged 23 years and unvaccinated against rabies, was bitten by a dog in Morocco in July 2004. One month later he was hospitalised in Ceuta with symptoms compatible with rabies. He died on 23 September in an Austrian hospital after a diagnosis of rabies was confirmed by FAT, IHC and RT-PCR (including sequencing) of the neck skin and the RT-PCR (including sequencing) of the pharyngeal swab. This Austrian case of laboratory confirmed rabies highlights the urgent need for reinforcement of the international recommendations for travel vaccinations.

Outbreak of clinical listeriosis in sheep: evaluation from possible contamination routes from feed to raw produce and humans.

We report the results of clinical and microbiological investigations on Listeria monocytogenes infections in a flock of 55 sheep and describe the implications for the safety of the raw milk and raw-milk cheeses produced in the on-farm dairy. The outbreak was caused by feeding grass silage, which was contaminated with 5 log10 CFU L. monocytogenes/g. Clinically, although having been fed from the same batch of silage, abortive (nine ewes), encephalitic (one ewe) and septicaemic (four ewes) forms of listeriosis were observed during the outbreak phase. As the starting point of feeding the contaminated silage was known we could calculate an incubation period of 18+/-2 and 26 days for the abortive and the encephalitic form of listeriosis, respectively. Pathologically, the septicaemic cases suffered from Listeria accumulation at comparable numbers in visceral organs but not in the brain. Only a single ewe developed central nervous symptoms and a rhomb-encephalitis was immunohistologically confirmed. In this case the infection proceeded from the nasal mucosa into the brain, with no infections of the liver, spleen and other visceral organs. Sampling of the cheese production chain, the farm environment and the persons living at the farm revealed the exposure of a farm-worker to an isolate genetically indistinguishable from the outbreak clone, obviously through the consumption of faecally contaminated bovine raw milk. The cheese under processing was free of Listeria because, as a result of intensive consultations, the farmer ensured a proper acidification of the cheese. The epidemiological findings suggest that food safety matters should be assessed in any case where infection of food-producing animals with potential human pathogens is observed.

A human case of travel-related rabies in Austria, September 2004.

A young male Austrian tourist, aged 23 years and unvaccinated against rabies, was bitten by a dog in Morocco in July 2004. One month later he was hospitalised in Ceuta with symptoms compatible with rabies. He died on 23 September in an Austrian hospital after a diagnosis of rabies was confirmed by FAT, IHC and RT-PCR (including sequencing) of the neck skin and the RT-PCR (including sequencing) of the pharyngeal swab. This Austrian case of laboratory confirmed rabies highlights the urgent need for reinforcement of the international recommendations for travel vaccinations.

Clinical and histopathological aspects of naturally occurring mastitis caused by Listeria monocytogenes in cattle and ewes.

Listeria monocytogenes was isolated from the milk of two cows and two sheep with mastitis in one quarter and one udder half. The animals were observed over a period of 2-12 months. Clinical examination of the udder, bacteriological examinations and determination of somatic cell counts of milk samples were performed monthly. All four cases suffered from a subclinical mastitis characterized by an elevated somatic cell count (0.8-10.1 x 10(6) cells/ml), a persistent shedding of Listeria and by a normal appearance of the milk. The animals did not show any systemic reaction, but all animals developed an atrophy of the infected mammary gland. Histological examinations revealed a chronic interstitial mastitis with diffuse infiltration of lymphocytes, plasma cells and macrophages. All internal organs showed no abnormalities, no Listeria could be isolated. Listeria could however be isolated from the affected mammary parenchyma and from the mammary lymph node. The results of the bacteriological examination could be confirmed by means of PCR. Using PFGE, all the isolates from the same animal were identical. Immunohistochemical examination of the ovine mammary glands achieved a very strong immunoreactivity for CD5 cells. The mode of infection and the reaction of the immune system's defense of the ovine udders are discussed.

Clinical and pathological features of the Porcine Ulcerative Dermatitis Syndrome (PUDS).

The nomenclature of ulcerative dermatitis as used in literature is somehow confusing because on the one hand this skin disorder is associated with bacterial growth and on the other hand it is a synonym for a chronic sporadic disease of adult sows with unknown aetiology. Thus, we propose the terminus 'Porcine Ulcerative Dermatitis Syndrome (PUDS)' for the latter to distinguish between these two disease complexes. This syndrome could be identified by clinical and pathological examinations in six sows, that were submitted to the clinic. Epidermal ulcers could be found nearly all over the body, but teats were always spared. Haematological examination showed a slight anaemia but physiological leucocyte counts. However, lymphopenia (x = 44.8%), granulocytosis (x = 42.0%) and an increased number of monocytes (x = 13.1%) could be found. Histologically, a lymphoplasmacytic and granulohistiocytic infiltration in the corium was most prominent. In some cases, a moderate leucocytoclastic vasculitis and perivasculitis could be seen at the dermo-epidermal border. Additionally, a multifocal interstitial nephritis with lymphoplasmacytic infiltration was a prominent feature in all animals. Participation of an immune complex associated disorder can be assumed when regarding histological findings as skin lesions in combination with glomerulonephritis are a common feature of such diseases. Also, IgG levels were elevated two- to fourfold in all affected sows when compared with healthy control pigs. This supports the hypothesis that not only T cells, as shown previously, but also the humoral branch of the immune system is involved in the aetiology of PUDS.

Abdominal mesothelioma in a goat.

CASE HISTORY: An 8-year-old female Toggenburg goat was presented with a history of reduced appetite, ruminal tympany, and abdominal enlargement of 2 weeks' duration. CLINICAL FINDINGS: The abdomen was markedly distended and contained a large amount of fluid, demonstrated using ultrasonography, along with echogenic masses on the ventral ruminal and reticular walls. The findings were confirmed at exploratory laparotomy, where together with excessive peritoneal fluid, numerous nodules were found blanketing the peritoneum. PATHOLOGICAL FINDINGS AND DIAGNOSIS: Numerous partially coalescing, white-grey nodules up to 3 cm in diameter covered the peritoneum. A biphasic mesothelioma consisting of epithelial and mesenchymal components was demonstrated by histopathology and immunohistochemistry. CLINICAL RELEVANCE: Primary tumours of the peritoneum are rare and occur in many animal species. They must be differentiated from chronic granulomatous peritonitis caused by bacteria (e.g. tuberculosis or 'pearl disease'), parasites and metastatic tumours.

[Isoimmune haemolytic icterus in neonatal calves as a consequence of vaccination against piroplasmosis]. / Isoimmunhämolytischer Ikterus bei Kälbern in Folge einer Piroplasmose-Schutzimpfung.

The article refers about several cases of isoimmunohaemolytic icterus in neonatal calves from different farms, whose dams had all been vaccinated against piroplasmosis. Clinical signs of immunomediated icterus neonatorum gravis, results of blood chemistry (with special regard to liver-specific parameters in the neonatal calf and results of haematology) as well as gross pathology and pathohistology are to be discussed. It is summarized, that the most relevant indicators for a hepatopathy in the newborn calf are total-bilirubin and the glutamate-dehydrogenase. Today, the production of piroplasmosis-vaccines out of blood of splenectomized animals is referred to as the only practicable method of harvesting sufficient amounts of vaccine-antigen.

[Calcinosis in goats]. / Kalzinose bei Ziegen.

Death, abortion, decreased milk yield and emaciation in dairy goats occurred due to calcinosis in a goat operation on 1100 meter sea level in Tyrol. This could be diagnosed by the clinical, sonographical and pathomorphological investigation. In the basic fodder yellow oat grass proportion was present with 40%. Clinically the apathic goats showed reduced appetite, emaciation, dyspnea, anaemic mucous membranes, heart noises and less activity to move. The blood-chemical examination of one goat revealed an increased activity of AP (199 U/l), calcium and phosphor-concentrations were in normal range. Sonographically liquidothorax and -perikard, calcified pulmonary and aortic valves and a thickened aortic orifice as well as calcification of kidneys, enlargment of liver and ascites could be diagnosed in this goat. The sonographical findings were confirmed by pathomorphological examination.